Construct: shRNA TRCN0000247019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053246.2-339s21c1
DNA Barcode:
AGCAGGAAGCTCGGGATTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dok4 (114255)
Gene Description:
docking protein 4
Transcript:
RefSeq NM_053246.2 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGGAAGCTCGGGATTTAC
Hairpin Sequence:
5'-CCGG-AGCAGGAAGCTCGGGATTTAC-CTCGAG-GTAAATCCCGAGCTTCCTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGGAAGCTCGGGATTTACCTCGAGGTAAATCCCGAGCTTCCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGGAAGCTCGGGATTTACCTCGAGGTAAATCCCGAGCTTCCTGCT-3'

Other clones with same target sequence:

(none)