Construct: shRNA TRCN0000247043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013747.4-1402s21c1
DNA Barcode:
ACACGGATACTCCAATCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golga5 (27277)
Gene Description:
golgi autoantigen, golgin subfamily a, 5
Transcript:
RefSeq NM_013747.4 (CURRENT)
Match location:
Position 1402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACGGATACTCCAATCTAAA
Hairpin Sequence:
5'-CCGG-ACACGGATACTCCAATCTAAA-CTCGAG-TTTAGATTGGAGTATCCGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACGGATACTCCAATCTAAACTCGAGTTTAGATTGGAGTATCCGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACGGATACTCCAATCTAAACTCGAGTTTAGATTGGAGTATCCGTGT-3'

Other clones with same target sequence:

(none)