Construct: shRNA TRCN0000247063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025905.2-683s21c1
DNA Barcode:
CTAGCGGAGGCGTATGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc23 (67009)
Gene Description:
tetratricopeptide repeat domain 23
Transcript:
RefSeq NM_025905.2 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGCGGAGGCGTATGTTAAT
Hairpin Sequence:
5'-CCGG-CTAGCGGAGGCGTATGTTAAT-CTCGAG-ATTAACATACGCCTCCGCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGCGGAGGCGTATGTTAATCTCGAGATTAACATACGCCTCCGCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGCGGAGGCGTATGTTAATCTCGAGATTAACATACGCCTCCGCTAG-3'

Other clones with same target sequence:

(none)