Construct: shRNA TRCN0000247142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025876.2-748s21c1
DNA Barcode:
GGATGAGACCTACGCAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk5rap1 (66971)
Gene Description:
CDK5 regulatory subunit associated protein 1
Transcript:
RefSeq NM_025876.2 (CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATGAGACCTACGCAGATAT
Hairpin Sequence:
5'-CCGG-GGATGAGACCTACGCAGATAT-CTCGAG-ATATCTGCGTAGGTCTCATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATGAGACCTACGCAGATATCTCGAGATATCTGCGTAGGTCTCATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATGAGACCTACGCAGATATCTCGAGATATCTGCGTAGGTCTCATCC-3'

Other clones with same target sequence:

(none)