Construct: shRNA TRCN0000247185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178605.4-229s21c1
DNA Barcode:
ATGCCTGGGACCACACCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nop16 (28126)
Gene Description:
NOP16 nucleolar protein
Transcript:
RefSeq NM_178605.4 (CURRENT)
Match location:
Position 229 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCCTGGGACCACACCAAAT
Hairpin Sequence:
5'-CCGG-ATGCCTGGGACCACACCAAAT-CTCGAG-ATTTGGTGTGGTCCCAGGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCCTGGGACCACACCAAATCTCGAGATTTGGTGTGGTCCCAGGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCCTGGGACCACACCAAATCTCGAGATTTGGTGTGGTCCCAGGCAT-3'

Other clones with same target sequence:

(none)