Construct: shRNA TRCN0000247235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026075.2-404s21c1
DNA Barcode:
TCTTACTCCAGTGCCACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srek1ip1 (67288)
Gene Description:
splicing regulatory glutamine/lysine-rich protein 1interacting protein 1
Transcript:
RefSeq NM_026075.2 (NON-CURRENT)
Match location:
Position 404 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTACTCCAGTGCCACTGAA
Hairpin Sequence:
5'-CCGG-TCTTACTCCAGTGCCACTGAA-CTCGAG-TTCAGTGGCACTGGAGTAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTACTCCAGTGCCACTGAACTCGAGTTCAGTGGCACTGGAGTAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTACTCCAGTGCCACTGAACTCGAGTTCAGTGGCACTGGAGTAAGA-3'

Other clones with same target sequence:

(none)