Construct: shRNA TRCN0000247261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081293.1-542s21c1
DNA Barcode:
CCATCCGTCTCTAAGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rprd2 (75137)
Gene Description:
regulation of nuclear pre-mRNA domain containing 2
Transcript:
RefSeq NM_001081293.1 (CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCCGTCTCTAAGTCTATA
Hairpin Sequence:
5'-CCGG-CCATCCGTCTCTAAGTCTATA-CTCGAG-TATAGACTTAGAGACGGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCCGTCTCTAAGTCTATACTCGAGTATAGACTTAGAGACGGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCCGTCTCTAAGTCTATACTCGAGTATAGACTTAGAGACGGATGG-3'

Other clones with same target sequence:

(none)