Construct: shRNA TRCN0000247272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177782.3-4583s21c1
DNA Barcode:
AGAAGGTGCAGCAGTACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prex1 (277360)
Gene Description:
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Transcript:
RefSeq NM_177782.3 (CURRENT)
Match location:
Position 4583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGGTGCAGCAGTACTATA
Hairpin Sequence:
5'-CCGG-AGAAGGTGCAGCAGTACTATA-CTCGAG-TATAGTACTGCTGCACCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGGTGCAGCAGTACTATACTCGAGTATAGTACTGCTGCACCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGGTGCAGCAGTACTATACTCGAGTATAGTACTGCTGCACCTTCT-3'

Other clones with same target sequence:

(none)