Construct: shRNA TRCN0000247276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012010.3-554s21c1
DNA Barcode:
TCATGAAACTCAAGCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2s3x (26905)
Gene Description:
eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked
Transcript:
RefSeq NM_012010.3 (CURRENT)
Match location:
Position 554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATGAAACTCAAGCATATTT
Hairpin Sequence:
5'-CCGG-TCATGAAACTCAAGCATATTT-CTCGAG-AAATATGCTTGAGTTTCATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATGAAACTCAAGCATATTTCTCGAGAAATATGCTTGAGTTTCATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATGAAACTCAAGCATATTTCTCGAGAAATATGCTTGAGTTTCATGA-3'

Other clones with same target sequence:

(none)