Construct: shRNA TRCN0000247462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138594.3-1410s21c1
DNA Barcode:
TGCTCGGGATCCCGCTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D6Wsu163e (28040)
Gene Description:
DNA segment, Chr 6, Wayne State University 163, expressed
Transcript:
RefSeq NM_138594.3 (CURRENT)
Match location:
Position 1410 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTCGGGATCCCGCTATTAT
Hairpin Sequence:
5'-CCGG-TGCTCGGGATCCCGCTATTAT-CTCGAG-ATAATAGCGGGATCCCGAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTCGGGATCCCGCTATTATCTCGAGATAATAGCGGGATCCCGAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTCGGGATCCCGCTATTATCTCGAGATAATAGCGGGATCCCGAGCA-3'

Other clones with same target sequence:

(none)