Construct: shRNA TRCN0000247527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030228.2-636s21c1
DNA Barcode:
ACGCCCAATGACCTTCGAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gas2l1 (78926)
Gene Description:
growth arrest-specific 2 like 1
Transcript:
RefSeq NM_030228.2 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCCCAATGACCTTCGAAAC
Hairpin Sequence:
5'-CCGG-ACGCCCAATGACCTTCGAAAC-CTCGAG-GTTTCGAAGGTCATTGGGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCCCAATGACCTTCGAAACCTCGAGGTTTCGAAGGTCATTGGGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCCCAATGACCTTCGAAACCTCGAGGTTTCGAAGGTCATTGGGCGT-3'

Other clones with same target sequence:

(none)