Construct: shRNA TRCN0000247534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173778.3-934s21c1
DNA Barcode:
ACTATGGCTATGAGAAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2d7 (244885)
Gene Description:
SH2 domain containing 7
Transcript:
RefSeq NM_173778.3 (CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTATGGCTATGAGAAGATTT
Hairpin Sequence:
5'-CCGG-ACTATGGCTATGAGAAGATTT-CTCGAG-AAATCTTCTCATAGCCATAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTATGGCTATGAGAAGATTTCTCGAGAAATCTTCTCATAGCCATAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTATGGCTATGAGAAGATTTCTCGAGAAATCTTCTCATAGCCATAGT-3'

Other clones with same target sequence:

(none)