Construct: shRNA TRCN0000247589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080495.2-2481s21c1
DNA Barcode:
TGTCGCTGAGTAACGTCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNRC18 (84629)
Gene Description:
trinucleotide repeat containing 18
Transcript:
RefSeq NM_001080495.2 (CURRENT)
Match location:
Position 2481 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCGCTGAGTAACGTCAAAG
Hairpin Sequence:
5'-CCGG-TGTCGCTGAGTAACGTCAAAG-CTCGAG-CTTTGACGTTACTCAGCGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCGCTGAGTAACGTCAAAGCTCGAGCTTTGACGTTACTCAGCGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCGCTGAGTAACGTCAAAGCTCGAGCTTTGACGTTACTCAGCGACA-3'

Other clones with same target sequence:

(none)