Construct: shRNA TRCN0000247591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080495.2-10159s21c1
DNA Barcode:
CTGACGTGCAGAATATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNRC18 (84629)
Gene Description:
trinucleotide repeat containing 18
Transcript:
RefSeq NM_001080495.2 (CURRENT)
Match location:
Position 10159 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACGTGCAGAATATTATTT
Hairpin Sequence:
5'-CCGG-CTGACGTGCAGAATATTATTT-CTCGAG-AAATAATATTCTGCACGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACGTGCAGAATATTATTTCTCGAGAAATAATATTCTGCACGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACGTGCAGAATATTATTTCTCGAGAAATAATATTCTGCACGTCAG-3'

Other clones with same target sequence:

(none)