Construct: shRNA TRCN0000247634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101372.1-2344s21c1
DNA Barcode:
TCCCAGGAGACCCTCTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGLON5 (402665)
Gene Description:
IgLON family member 5
Transcript:
RefSeq NM_001101372.1 (NON-CURRENT)
Match location:
Position 2344 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCAGGAGACCCTCTATATA
Hairpin Sequence:
5'-CCGG-TCCCAGGAGACCCTCTATATA-CTCGAG-TATATAGAGGGTCTCCTGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCAGGAGACCCTCTATATACTCGAGTATATAGAGGGTCTCCTGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCAGGAGACCCTCTATATACTCGAGTATATAGAGGGTCTCCTGGGA-3'

Other clones with same target sequence:

(none)