Construct: shRNA TRCN0000247713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080527.1-5713s21c1
DNA Barcode:
AGGTGTACTTCATGCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO7B (4648)
Gene Description:
myosin VIIB
Transcript:
RefSeq NM_001080527.1 (CURRENT)
Match location:
Position 5713 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGTACTTCATGCGGAAAT
Hairpin Sequence:
5'-CCGG-AGGTGTACTTCATGCGGAAAT-CTCGAG-ATTTCCGCATGAAGTACACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGTACTTCATGCGGAAATCTCGAGATTTCCGCATGAAGTACACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGTACTTCATGCGGAAATCTCGAGATTTCCGCATGAAGTACACCT-3'

Other clones with same target sequence:

(none)