Construct: shRNA TRCN0000247716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175710.1-631s21c1
DNA Barcode:
TAGCATCAGCAGAGAGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CR1L (1379)
Gene Description:
complement C3b/C4b receptor 1 like
Transcript:
RefSeq NM_175710.1 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCATCAGCAGAGAGTATTT
Hairpin Sequence:
5'-CCGG-TAGCATCAGCAGAGAGTATTT-CTCGAG-AAATACTCTCTGCTGATGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCATCAGCAGAGAGTATTTCTCGAGAAATACTCTCTGCTGATGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCATCAGCAGAGAGTATTTCTCGAGAAATACTCTCTGCTGATGCTA-3'

Other clones with same target sequence:

(none)