Construct: shRNA TRCN0000247733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024174.5-225s21c1
DNA Barcode:
GGATCAGATTCGAGCGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps23 (64656)
Gene Description:
mitochondrial ribosomal protein S23
Transcript:
RefSeq NM_024174.5 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCAGATTCGAGCGAAATT
Hairpin Sequence:
5'-CCGG-GGATCAGATTCGAGCGAAATT-CTCGAG-AATTTCGCTCGAATCTGATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCAGATTCGAGCGAAATTCTCGAGAATTTCGCTCGAATCTGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCAGATTCGAGCGAAATTCTCGAGAATTTCGCTCGAATCTGATCC-3'

Other clones with same target sequence:

(none)