Construct: shRNA TRCN0000247755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146205.2-3556s21c1
DNA Barcode:
TATGCCCTAGGCTAAACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc5 (233912)
Gene Description:
armadillo repeat containing 5
Transcript:
RefSeq NM_146205.2 (CURRENT)
Match location:
Position 3556 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGCCCTAGGCTAAACTATT
Hairpin Sequence:
5'-CCGG-TATGCCCTAGGCTAAACTATT-CTCGAG-AATAGTTTAGCCTAGGGCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGCCCTAGGCTAAACTATTCTCGAGAATAGTTTAGCCTAGGGCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGCCCTAGGCTAAACTATTCTCGAGAATAGTTTAGCCTAGGGCATA-3'

Other clones with same target sequence:

(none)