Construct: shRNA TRCN0000247777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144796.4-608s21c1
DNA Barcode:
TGTACAACCTGGTTTCGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Susd4 (96935)
Gene Description:
sushi domain containing 4
Transcript:
RefSeq NM_144796.4 (CURRENT)
Match location:
Position 608 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTACAACCTGGTTTCGTTAT
Hairpin Sequence:
5'-CCGG-TGTACAACCTGGTTTCGTTAT-CTCGAG-ATAACGAAACCAGGTTGTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTACAACCTGGTTTCGTTATCTCGAGATAACGAAACCAGGTTGTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTACAACCTGGTTTCGTTATCTCGAGATAACGAAACCAGGTTGTACA-3'

Other clones with same target sequence:

(none)