Construct: shRNA TRCN0000247788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040111.1-5265s21c1
DNA Barcode:
GCATGTTTACTGAGCTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arap1 (69710)
Gene Description:
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Transcript:
RefSeq NM_001040111.1 (CURRENT)
Match location:
Position 5265 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTTACTGAGCTATTTA
Hairpin Sequence:
5'-CCGG-GCATGTTTACTGAGCTATTTA-CTCGAG-TAAATAGCTCAGTAAACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTTACTGAGCTATTTACTCGAGTAAATAGCTCAGTAAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTTACTGAGCTATTTACTCGAGTAAATAGCTCAGTAAACATGC-3'

Other clones with same target sequence:

(none)