Construct: shRNA TRCN0000247800
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_138584.2-2111s21c1
- DNA Barcode:
- CCTATCCTGGATGGCTATAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Spg21 (27965)
- Gene Description:
- SPG21, maspardin
- Transcript:
- RefSeq NM_138584.2 (CURRENT)
- Match location:
- Position 2111 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000247800)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTATCCTGGATGGCTATAAA
- Hairpin Sequence:
-
5'-CCGG-CCTATCCTGGATGGCTATAAA-CTCGAG-TTTATAGCCATCCAGGATAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTATCCTGGATGGCTATAAACTCGAGTTTATAGCCATCCAGGATAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTATCCTGGATGGCTATAAACTCGAGTTTATAGCCATCCAGGATAGG-3'
Other clones with same target sequence:
(none)
