Construct: shRNA TRCN0000247819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172945.2-1018s21c1
DNA Barcode:
GGCAAAGTCAAAGGTTGTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd13b (268445)
Gene Description:
ankyrin repeat domain 13b
Transcript:
RefSeq NM_172945.2 (CURRENT)
Match location:
Position 1018 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAAAGTCAAAGGTTGTAAG
Hairpin Sequence:
5'-CCGG-GGCAAAGTCAAAGGTTGTAAG-CTCGAG-CTTACAACCTTTGACTTTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAAAGTCAAAGGTTGTAAGCTCGAGCTTACAACCTTTGACTTTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAAAGTCAAAGGTTGTAAGCTCGAGCTTACAACCTTTGACTTTGCC-3'

Other clones with same target sequence:

(none)