Construct: shRNA TRCN0000247889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029948.2-1865s21c1
DNA Barcode:
CTTCGGGCTATAGGGTAAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pramef12 (77632)
Gene Description:
PRAME family member 12
Transcript:
RefSeq NM_029948.2 (CURRENT)
Match location:
Position 1865 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGGGCTATAGGGTAAATG
Hairpin Sequence:
5'-CCGG-CTTCGGGCTATAGGGTAAATG-CTCGAG-CATTTACCCTATAGCCCGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGGGCTATAGGGTAAATGCTCGAGCATTTACCCTATAGCCCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGGGCTATAGGGTAAATGCTCGAGCATTTACCCTATAGCCCGAAG-3'

Other clones with same target sequence:

(none)