Construct: shRNA TRCN0000247896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029291.1-137s21c1
DNA Barcode:
GCAGAAGGCAGACCGGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ascc2 (75452)
Gene Description:
activating signal cointegrator 1 complex subunit 2
Transcript:
RefSeq NM_029291.1 (CURRENT)
Match location:
Position 137 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGGCAGACCGGTATTT
Hairpin Sequence:
5'-CCGG-GCAGAAGGCAGACCGGTATTT-CTCGAG-AAATACCGGTCTGCCTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGGCAGACCGGTATTTCTCGAGAAATACCGGTCTGCCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGGCAGACCGGTATTTCTCGAGAAATACCGGTCTGCCTTCTGC-3'

Other clones with same target sequence:

(none)