Construct: shRNA TRCN0000247901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011573.2-591s21c1
DNA Barcode:
TGCTGGCCTTTGCTGGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tex264 (21767)
Gene Description:
testis expressed gene 264
Transcript:
RefSeq NM_011573.2 (NON-CURRENT)
Match location:
Position 591 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000247901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGGCCTTTGCTGGTTATT
Hairpin Sequence:
5'-CCGG-TGCTGGCCTTTGCTGGTTATT-CTCGAG-AATAACCAGCAAAGGCCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGGCCTTTGCTGGTTATTCTCGAGAATAACCAGCAAAGGCCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGGCCTTTGCTGGTTATTCTCGAGAATAACCAGCAAAGGCCAGCA-3'

Other clones with same target sequence:

(none)