Construct: shRNA TRCN0000248027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178911.4-1617s21c1
DNA Barcode:
CTGAGGGTTGATCCCTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pld4 (104759)
Gene Description:
phospholipase D family, member 4
Transcript:
RefSeq NM_178911.4 (CURRENT)
Match location:
Position 1617 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGGGTTGATCCCTTTGAT
Hairpin Sequence:
5'-CCGG-CTGAGGGTTGATCCCTTTGAT-CTCGAG-ATCAAAGGGATCAACCCTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGGGTTGATCCCTTTGATCTCGAGATCAAAGGGATCAACCCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGGGTTGATCCCTTTGATCTCGAGATCAAAGGGATCAACCCTCAG-3'

Other clones with same target sequence:

(none)