Construct: shRNA TRCN0000248049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178358.3-777s21c1
DNA Barcode:
CGGGAATGTCTCCAATCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lhfpl1 (237091)
Gene Description:
lipoma HMGIC fusion partner-like 1
Transcript:
RefSeq NM_178358.3 (CURRENT)
Match location:
Position 777 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAATGTCTCCAATCAATT
Hairpin Sequence:
5'-CCGG-CGGGAATGTCTCCAATCAATT-CTCGAG-AATTGATTGGAGACATTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAATGTCTCCAATCAATTCTCGAGAATTGATTGGAGACATTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAATGTCTCCAATCAATTCTCGAGAATTGATTGGAGACATTCCCG-3'

Other clones with same target sequence:

(none)