Construct: shRNA TRCN0000248159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139306.1-2596s21c1
DNA Barcode:
CTCGGGAGCTGCCCTTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acer2 (230379)
Gene Description:
alkaline ceramidase 2
Transcript:
RefSeq NM_139306.1 (NON-CURRENT)
Match location:
Position 2596 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGGAGCTGCCCTTATATA
Hairpin Sequence:
5'-CCGG-CTCGGGAGCTGCCCTTATATA-CTCGAG-TATATAAGGGCAGCTCCCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGGAGCTGCCCTTATATACTCGAGTATATAAGGGCAGCTCCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGGAGCTGCCCTTATATACTCGAGTATATAAGGGCAGCTCCCGAG-3'

Other clones with same target sequence:

(none)