Construct: shRNA TRCN0000248243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001128086.1-3505s21c1
DNA Barcode:
ATTTATCGATGCGGCAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anks1b (77531)
Gene Description:
ankyrin repeat and sterile alpha motif domain containing 1B
Transcript:
RefSeq NM_001128086.1 (NON-CURRENT)
Match location:
Position 3505 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTATCGATGCGGCAAATAA
Hairpin Sequence:
5'-CCGG-ATTTATCGATGCGGCAAATAA-CTCGAG-TTATTTGCCGCATCGATAAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTATCGATGCGGCAAATAACTCGAGTTATTTGCCGCATCGATAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTATCGATGCGGCAAATAACTCGAGTTATTTGCCGCATCGATAAAT-3'

Other clones with same target sequence:

(none)