Construct: shRNA TRCN0000248337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018878.2-3122s21c1
DNA Barcode:
ACTTATGTCGCGAGTACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Paxip1 (55982)
Gene Description:
PAX interacting (with transcription-activation domain) protein 1
Transcript:
RefSeq NM_018878.2 (NON-CURRENT)
Match location:
Position 3122 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTATGTCGCGAGTACTTTG
Hairpin Sequence:
5'-CCGG-ACTTATGTCGCGAGTACTTTG-CTCGAG-CAAAGTACTCGCGACATAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTATGTCGCGAGTACTTTGCTCGAGCAAAGTACTCGCGACATAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTATGTCGCGAGTACTTTGCTCGAGCAAAGTACTCGCGACATAAGT-3'

Other clones with same target sequence:

(none)