Construct: shRNA TRCN0000248383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178773.4-2449s21c1
DNA Barcode:
GAACTTGGCTACCCGTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ano4 (320091)
Gene Description:
anoctamin 4
Transcript:
RefSeq NM_178773.4 (NON-CURRENT)
Match location:
Position 2449 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTTGGCTACCCGTTAATT
Hairpin Sequence:
5'-CCGG-GAACTTGGCTACCCGTTAATT-CTCGAG-AATTAACGGGTAGCCAAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTTGGCTACCCGTTAATTCTCGAGAATTAACGGGTAGCCAAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTTGGCTACCCGTTAATTCTCGAGAATTAACGGGTAGCCAAGTTC-3'

Other clones with same target sequence:

(none)