Construct: shRNA TRCN0000248411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023873.3-2254s21c1
DNA Barcode:
AGTACAGAAAGTCGCAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep70 (68121)
Gene Description:
centrosomal protein 70
Transcript:
RefSeq NM_023873.3 (CURRENT)
Match location:
Position 2254 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTACAGAAAGTCGCAGTTAA
Hairpin Sequence:
5'-CCGG-AGTACAGAAAGTCGCAGTTAA-CTCGAG-TTAACTGCGACTTTCTGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACAGAAAGTCGCAGTTAACTCGAGTTAACTGCGACTTTCTGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACAGAAAGTCGCAGTTAACTCGAGTTAACTGCGACTTTCTGTACT-3'

Other clones with same target sequence:

(none)