Construct: shRNA TRCN0000248452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145960.3-1586s21c1
DNA Barcode:
CGTATGAAGTTCGTGACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtrf1 (211253)
Gene Description:
mitochondrial translational release factor 1
Transcript:
RefSeq NM_145960.3 (NON-CURRENT)
Match location:
Position 1586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATGAAGTTCGTGACATTA
Hairpin Sequence:
5'-CCGG-CGTATGAAGTTCGTGACATTA-CTCGAG-TAATGTCACGAACTTCATACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATGAAGTTCGTGACATTACTCGAGTAATGTCACGAACTTCATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATGAAGTTCGTGACATTACTCGAGTAATGTCACGAACTTCATACG-3'

Other clones with same target sequence:

  1. TRCN0000200636