Construct: shRNA TRCN0000248454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026470.3-811s21c1
DNA Barcode:
ACTACGAACAGCCTACGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata6 (67946)
Gene Description:
spermatogenesis associated 6
Transcript:
RefSeq NM_026470.3 (CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTACGAACAGCCTACGATTT
Hairpin Sequence:
5'-CCGG-ACTACGAACAGCCTACGATTT-CTCGAG-AAATCGTAGGCTGTTCGTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACGAACAGCCTACGATTTCTCGAGAAATCGTAGGCTGTTCGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACGAACAGCCTACGATTTCTCGAGAAATCGTAGGCTGTTCGTAGT-3'

Other clones with same target sequence:

(none)