Construct: shRNA TRCN0000248458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024474.2-1228s21c1
DNA Barcode:
GGTCCTTATCCTTGAGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col26a1 (140709)
Gene Description:
collagen, type XXVI, alpha 1
Transcript:
RefSeq NM_024474.2 (CURRENT)
Match location:
Position 1228 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCCTTATCCTTGAGCATAT
Hairpin Sequence:
5'-CCGG-GGTCCTTATCCTTGAGCATAT-CTCGAG-ATATGCTCAAGGATAAGGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCCTTATCCTTGAGCATATCTCGAGATATGCTCAAGGATAAGGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCCTTATCCTTGAGCATATCTCGAGATATGCTCAAGGATAAGGACC-3'

Other clones with same target sequence:

(none)