Construct: shRNA TRCN0000248536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030132.4-369s21c1
DNA Barcode:
CCACACAGGATCAGAACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Utp23 (78581)
Gene Description:
UTP23 small subunit processome component
Transcript:
RefSeq NM_030132.4 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACACAGGATCAGAACTTAT
Hairpin Sequence:
5'-CCGG-CCACACAGGATCAGAACTTAT-CTCGAG-ATAAGTTCTGATCCTGTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACACAGGATCAGAACTTATCTCGAGATAAGTTCTGATCCTGTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACACAGGATCAGAACTTATCTCGAGATAAGTTCTGATCCTGTGTGG-3'

Other clones with same target sequence:

(none)