Construct: shRNA TRCN0000248551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011825.1-669s21c1
DNA Barcode:
GACCCACCTTTCCGAATCAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grem2 (23893)
Gene Description:
gremlin 2, DAN family BMP antagonist
Transcript:
RefSeq NM_011825.1 (CURRENT)
Match location:
Position 669 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCACCTTTCCGAATCAAG
Hairpin Sequence:
5'-CCGG-GACCCACCTTTCCGAATCAAG-CTCGAG-CTTGATTCGGAAAGGTGGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCACCTTTCCGAATCAAGCTCGAGCTTGATTCGGAAAGGTGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCACCTTTCCGAATCAAGCTCGAGCTTGATTCGGAAAGGTGGGTC-3'

Other clones with same target sequence:

(none)