Construct: shRNA TRCN0000248631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198162.1-849s21c1
DNA Barcode:
GACTCTCCTGCTGCCTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Morc2a (74522)
Gene Description:
microrchidia 2A
Transcript:
RefSeq NM_198162.1 (CURRENT)
Match location:
Position 849 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCTCCTGCTGCCTATATA
Hairpin Sequence:
5'-CCGG-GACTCTCCTGCTGCCTATATA-CTCGAG-TATATAGGCAGCAGGAGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCTCCTGCTGCCTATATACTCGAGTATATAGGCAGCAGGAGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCTCCTGCTGCCTATATACTCGAGTATATAGGCAGCAGGAGAGTC-3'

Other clones with same target sequence:

(none)