Construct: shRNA TRCN0000248668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178628.4-1710s21c1
DNA Barcode:
ATCCGCTACTCTGGCGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atl1 (73991)
Gene Description:
atlastin GTPase 1
Transcript:
RefSeq NM_178628.4 (NON-CURRENT)
Match location:
Position 1710 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGCTACTCTGGCGAATAT
Hairpin Sequence:
5'-CCGG-ATCCGCTACTCTGGCGAATAT-CTCGAG-ATATTCGCCAGAGTAGCGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGCTACTCTGGCGAATATCTCGAGATATTCGCCAGAGTAGCGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGCTACTCTGGCGAATATCTCGAGATATTCGCCAGAGTAGCGGAT-3'

Other clones with same target sequence:

(none)