Construct: shRNA TRCN0000248689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019514.3-2352s21c1
DNA Barcode:
CAACCGGACACAACATGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Astn2 (56079)
Gene Description:
astrotactin 2
Transcript:
RefSeq NM_019514.3 (CURRENT)
Match location:
Position 2352 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGGACACAACATGTAAA
Hairpin Sequence:
5'-CCGG-CAACCGGACACAACATGTAAA-CTCGAG-TTTACATGTTGTGTCCGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGGACACAACATGTAAACTCGAGTTTACATGTTGTGTCCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGGACACAACATGTAAACTCGAGTTTACATGTTGTGTCCGGTTG-3'

Other clones with same target sequence:

(none)