Construct: shRNA TRCN0000248705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172676.2-581s21c1
DNA Barcode:
GCTTCGACTGAATGCAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samd10 (229011)
Gene Description:
sterile alpha motif domain containing 10
Transcript:
RefSeq NM_172676.2 (CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCGACTGAATGCAGATAA
Hairpin Sequence:
5'-CCGG-GCTTCGACTGAATGCAGATAA-CTCGAG-TTATCTGCATTCAGTCGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCGACTGAATGCAGATAACTCGAGTTATCTGCATTCAGTCGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCGACTGAATGCAGATAACTCGAGTTATCTGCATTCAGTCGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000216902