Construct: shRNA TRCN0000248800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198013.2-1578s21c1
DNA Barcode:
ACCAAGGCTCAGGCTTCTTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cuedc1 (103841)
Gene Description:
CUE domain containing 1
Transcript:
RefSeq NM_198013.2 (NON-CURRENT)
Match location:
Position 1578 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAAGGCTCAGGCTTCTTCA
Hairpin Sequence:
5'-CCGG-ACCAAGGCTCAGGCTTCTTCA-CTCGAG-TGAAGAAGCCTGAGCCTTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAAGGCTCAGGCTTCTTCACTCGAGTGAAGAAGCCTGAGCCTTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAAGGCTCAGGCTTCTTCACTCGAGTGAAGAAGCCTGAGCCTTGGT-3'

Other clones with same target sequence:

(none)