Construct: shRNA TRCN0000248890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177592.3-833s21c1
DNA Barcode:
CTACCGTCATCTTCAAGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem164 (209497)
Gene Description:
transmembrane protein 164
Transcript:
RefSeq NM_177592.3 (NON-CURRENT)
Match location:
Position 833 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCGTCATCTTCAAGTTAC
Hairpin Sequence:
5'-CCGG-CTACCGTCATCTTCAAGTTAC-CTCGAG-GTAACTTGAAGATGACGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCGTCATCTTCAAGTTACCTCGAGGTAACTTGAAGATGACGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCGTCATCTTCAAGTTACCTCGAGGTAACTTGAAGATGACGGTAG-3'

Other clones with same target sequence:

(none)