Construct: shRNA TRCN0000248969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145964.2-1709s21c1
DNA Barcode:
GCAAGAGATGACAGGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap1ar (211556)
Gene Description:
adaptor-related protein complex 1 associated regulatory protein
Transcript:
RefSeq NM_145964.2 (CURRENT)
Match location:
Position 1709 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAGATGACAGGATTAAA
Hairpin Sequence:
5'-CCGG-GCAAGAGATGACAGGATTAAA-CTCGAG-TTTAATCCTGTCATCTCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAGATGACAGGATTAAACTCGAGTTTAATCCTGTCATCTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAGATGACAGGATTAAACTCGAGTTTAATCCTGTCATCTCTTGC-3'

Other clones with same target sequence:

(none)