Construct: shRNA TRCN0000248993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178811.3-426s21c1
DNA Barcode:
GTCTCACAACTTCGGAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trappc12 (217449)
Gene Description:
trafficking protein particle complex 12
Transcript:
RefSeq NM_178811.3 (NON-CURRENT)
Match location:
Position 426 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCACAACTTCGGAGATAA
Hairpin Sequence:
5'-CCGG-GTCTCACAACTTCGGAGATAA-CTCGAG-TTATCTCCGAAGTTGTGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCACAACTTCGGAGATAACTCGAGTTATCTCCGAAGTTGTGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCACAACTTCGGAGATAACTCGAGTTATCTCCGAAGTTGTGAGAC-3'

Other clones with same target sequence:

(none)