Construct: shRNA TRCN0000248998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028360.2-429s21c1
DNA Barcode:
ATGACCTGATGGCCAACTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc19 (72795)
Gene Description:
tetratricopeptide repeat domain 19
Transcript:
RefSeq NM_028360.2 (CURRENT)
Match location:
Position 429 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000248998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACCTGATGGCCAACTTAG
Hairpin Sequence:
5'-CCGG-ATGACCTGATGGCCAACTTAG-CTCGAG-CTAAGTTGGCCATCAGGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACCTGATGGCCAACTTAGCTCGAGCTAAGTTGGCCATCAGGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACCTGATGGCCAACTTAGCTCGAGCTAAGTTGGCCATCAGGTCAT-3'

Other clones with same target sequence:

(none)