Construct: shRNA TRCN0000249021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019427.2-1169s21c1
DNA Barcode:
TCAGTGGACGGACGGAATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epb41l4b (54357)
Gene Description:
erythrocyte membrane protein band 4.1 like 4b
Transcript:
RefSeq NM_019427.2 (CURRENT)
Match location:
Position 1169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGTGGACGGACGGAATATC
Hairpin Sequence:
5'-CCGG-TCAGTGGACGGACGGAATATC-CTCGAG-GATATTCCGTCCGTCCACTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGTGGACGGACGGAATATCCTCGAGGATATTCCGTCCGTCCACTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGTGGACGGACGGAATATCCTCGAGGATATTCCGTCCGTCCACTGA-3'

Other clones with same target sequence:

(none)