Construct: shRNA TRCN0000249046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144895.1-2174s21c1
DNA Barcode:
GCGTCTTCTGTCTGTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spg20 (229285)
Gene Description:
spastic paraplegia 20, spartin (Troyer syndrome) homolog (human)
Transcript:
RefSeq NM_144895.1 (NON-CURRENT)
Match location:
Position 2174 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTTCTGTCTGTTCATTT
Hairpin Sequence:
5'-CCGG-GCGTCTTCTGTCTGTTCATTT-CTCGAG-AAATGAACAGACAGAAGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTTCTGTCTGTTCATTTCTCGAGAAATGAACAGACAGAAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTTCTGTCTGTTCATTTCTCGAGAAATGAACAGACAGAAGACGC-3'

Other clones with same target sequence:

  1. TRCN0000179319