Construct: shRNA TRCN0000249104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009048.2-2267s21c1
DNA Barcode:
GGCATCCATTAGACGAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Reps1 (19707)
Gene Description:
RalBP1 associated Eps domain containing protein
Transcript:
RefSeq NM_009048.2 (CURRENT)
Match location:
Position 2267 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000249104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCATCCATTAGACGAAATAA
Hairpin Sequence:
5'-CCGG-GGCATCCATTAGACGAAATAA-CTCGAG-TTATTTCGTCTAATGGATGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCATCCATTAGACGAAATAACTCGAGTTATTTCGTCTAATGGATGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCATCCATTAGACGAAATAACTCGAGTTATTTCGTCTAATGGATGCC-3'

Other clones with same target sequence:

  1. TRCN0000217553